A quantitative (stereological) study of the effects of experimental unilateral cryptorchidism and subsequent orchiopexy on spermatogenesis in adult rabbit testis.
نویسندگان
چکیده
The aim of this study was to examine the controversial effects of experimental unilateral cryptorchidism and subsequent orchiopexy on the number of germ cells and other morphometric characteristics of testicular and epididymal structures in adult rabbits. Unilateral cryptorchidism was induced in 11 mature male New Zealand white rabbits by returning one testis, together with the ipsilateral epididymis, to the abdominal cavity via a surgical procedure. After 3 months, testes and epididymides were removed from six animals (and from six age-matched control animals that did not undergo the surgery). Orchiopexy was performed on the five remaining animals and the testes and epididymides of these animals (and an additional six age-matched control animals) were removed 7 weeks later. A contemporary, unbiased and efficient stereological tool, the optical disector, was used to estimate the number of nuclei in the testis and epididymis using methacrylate-embedded sections of 25 micron in thickness. Cryptorchidism resulted in severe testicular atrophy and spermatogenic arrest: type A spermatogonia and Sertoli cells only were seen in the seminiferous epithelium, and the number of type A spermatogonia per testis was reduced by 84%. After orchiopexy, the testis remained atrophied and the number of type A spermatogonia returned to the near-normal range in four of five animals, but spermatogenesis was recovered only partially at the stage of early primary spermatocytes (one animal), late primary spermatocytes (two animals) or spermatids (one animal). In conclusion, cryptorchidism caused severe spermatogenic arrest that was potentially recoverable (in view of the restoration of the number of type A spermatogonia), but orchiopexy failed to induce full recovery of spermatogenesis.
منابع مشابه
Investigation of the Protective Effects of Amlodipine on the Structure and Function of Testicular Tissue following Experimental Unilateral Cryptorchidism in Rats
Objective- Cryptorchidism, common birth defect of the male genital tract, is one of the causes of fertility problems. The elevation of tissue temperature following of cryptorchidism could induce oxidative stress which influences the cellular and tissue degeneration. Amlodipine is a third-generation of calcium channel blockers which has antioxidant activity. The aim of this stud...
متن کاملEstablishment of a surgically induced cryptorchidism canine recipient model for spermatogonial stem cell transplantation
Transplantation of spermatogonial stem cells (SSCs) in experimental animal models has been used to study germ line stem cell biology and to produce transgenic animals. The species-specific recipient model preparation is important for the characterization of SSCs and the production of offspring. Here, we investigated the effects of surgically induced cryptorchidism in dog as a new recipient mode...
متن کاملEffect of Vitamin C on Epididymal Sperm Quality in the Rat Experimentally Induced Unilateral Cryptorchidism
Objective- Release of free radicals and oxidants following increased temperature of testis in cryptorchidism, play an important role in the producing testicular damage. The present study investigated the role of vitamin C as an antioxidant in protecting the testis against damage in experimental unilateral cryptorchidism. Design- Experimental study Animals- Forty five male adult Wistar–Albino ...
متن کاملZinc Therapy Improves Deleterious Effects of Experimental Unilateral Cryptorchidism: Histopathological Evaluation of Testes
Objective- Perturbation of the normal process of testicular descent results in the condition of cryptorchidism. Spermatogenesis is generally impaired in cryptorchid testes because of high intratesticular temperature. It seems that the main mechanisms underlying the process of spermatozoa demise is apoptosis by overgeneration of free radicals. So, the aim of the present study was to investigate ...
متن کاملO-38: Evaluation the Expression of Bax, Bcl-2,p53 & Survivine after Transplantation of SpermatogonialStem Cells to Cryptorchid MouseModel
Background: Disruption in spermatogenesis and infertility occurred after local testicular heating. In this study in addition to evaluation the long term effects of experimental bilateral and unilateral cryptorchidism on sperm parameters and structure of mouse testis, the genes that involved in programmed cell death after cryptorchidism and treatment methods of decreasing the germ cells in semin...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Reproduction
دوره 124 1 شماره
صفحات -
تاریخ انتشار 2002